Catalog Number - 68304-15P-4-3M

Cogent RP C4 Butyl™ Wide Pore is a reversed-phase HPLC stationary phase specifically designed for the separation of proteins, peptides, enzymes, and other large biomolecules. Manufactured using a traditional butyl (C4) bonded phase on ultra-high purity silica and fully double end capped, this 300 Å wide pore chemistry provides enhanced accessibility for larger molecules compared to standard 100 Å pore columns. The wide pore structure improves mass transfer and interaction with the stationary phase, making it an excellent choice for bioanalytical, biopharmaceutical, and biomolecule characterization applications.

68304-15P-4-3M is an HPLC column packed with 3 µm RP C4 Butyl™ Wide Pore particles featuring a 300 Å pore size in a 4.0 mm ID x 150 mm format. The 150 mm length provides maximum resolution and peak capacity for complex biomolecule separations, while the 3 µm particles deliver greater efficiency and sharper peaks than comparable 5 µm packings. Typical operating flow rates are approximately 0.8–1.5 mL/min depending on mobile phase composition, temperature, and instrument configuration.

This column is particularly well suited for proteins, peptides, enzymes, and other large hydrophobic or neutral biomolecules analyzed by reversed-phase HPLC. The 300 Å pore structure allows large molecules to fully access the bonded phase, improving recovery and separation performance. The C4 chemistry provides lower hydrophobic retention than C8 phases, which is often advantageous for biomolecules that may be excessively retained on more hydrophobic stationary phases. The 150 mm length maximizes resolving power for demanding biopharmaceutical and bioanalytical applications.

Key Benefits

• RP C4 Butyl™ Wide Pore stationary phase

• Fully double end capped

• Traditional butyl (C4) bonded phase

• Ultra-high purity silica support

• 300 Å wide pore design

• Optimized for proteins and peptides

• 3 µm particle technology

• Greater efficiency than 5 µm columns

• Maximum peak capacity

• Excellent for bioanalytical methods

Typical Applications

• Protein analysis

• Peptide analysis

• Enzyme characterization

• Bioanalytical methods

• Biopharmaceutical analysis

• Protein purity testing

• Peptide mapping

• Biomolecule separations

• Method development

• Complex sample characterization

Specifications

• Product Type: HPLC Column

• Stationary Phase: RP C4 Butyl™ Wide Pore

• Chromatography Mode: Reversed Phase

• Bonded Phase: Butyl (C4)

• Endcapping: Fully Double End Capped

• Particle Size: 3 µm

• Pore Size: 300 Å

• Column Dimensions: 4.0 mm ID x 150 mm

• Length: 150 mm

• Typical Flow Rate: 0.8–1.5 mL/min

• Brand: Cogent

Special Order Item Information

This is a Special Order Item manufactured to customer requirements and cannot be canceled or returned once production has begun.

For complete details, review the Custom Order Information and Ordering Policy.

For quotation requests, lead times, availability, or application assistance, please contact MICROSOLV Customer Service.

Important Notes

• Designed for proteins, peptides, enzymes, and other large biomolecules

• 300 Å wide pores improve access to the bonded phase for larger molecules

• Suitable for conventional HPLC systems

• 3 µm particles provide higher efficiency and sharper peaks than comparable 5 µm columns

• Generates higher backpressure than larger particle columns

• The 150 mm length maximizes resolution and peak capacity for complex biomolecule separations

• Lower retention than comparable C8 stationary phases

• Excellent choice for biopharmaceutical and biotechnology laboratories

• This is a Special Order Item